【作者】 李峰；

【导师】 陈主初；

【作者基本信息】 中南大学 ， 病理生理学， 2006， 博士

【摘要】 鼻咽癌是我国南方常见的恶性肿瘤之一。鼻咽癌在西方国家是一种罕见的恶性肿瘤，其发病率不足1／10万人年，而在我国南方其发病率高达25-30／10万人年，并且中国南方移民的发病风险亦远较西方本地人为高。因此，鼻咽癌明显的种族和地域分布的特点提示鼻咽癌是一种与遗传和环境因素密切相关的疾病。以往研究大多集中在单个或少数几个相关基因及其蛋白质产物上，难以反映鼻咽癌DNA改变的全貌。近年来也采用一些新技术如比较基因组杂交、微阵列等从DNA或mRNA整体水平进行了研究，但由于DNA或mRNA的改变仍不能完全反映细胞内蛋白质的变化情况，并且蛋白质还存在翻译后修饰、转运定位、构象变化以及蛋白质与蛋白质或其他生物分子之间相互作用等一系列自身特有的问题，因此若能直接从蛋白质整体入手来研究鼻咽癌，并与鼻咽癌中DNA和mRNA的改变情况相印证，将更全面、真实地揭示鼻咽癌变的过程和本质。 采用Sample Grinding Kit组织研磨试剂盒和组织裂解液改进了鼻咽癌这种小样本的组织抽提方法，结果表明用Sample Grinding Kit组织研磨试剂盒能有效增加总蛋白质的抽提得率，减少了不必要的样品损失，从而能最大限度地利用鼻咽癌组织。首次以人鼻咽癌组织为研究对象，应用双向凝胶电泳技术建立了分辨率高、重复性好的人鼻咽癌组织的双向凝胶电泳图谱，并采用肽质量指纹和肽序列标签两种质谱技术对双向凝胶电泳图更多还原

【Abstract】 Nasopharyngeal carcinoma (NPC) is one of the most common cancers in southern China and Southeast Asia. This cancer is prevalent in the southern part of China including Guandoing and Hunan Provinces (25-50/100,000) but less so among in western country, where incidence of NPC is less than 1/100,000, and the immigrant from southern China have more risk of NPC than native. The marked geographic and racial differences in incidence of NPC indicate that the development of this cancer must be related to special genetic and environmental factors. To date, most research about NPC were focused on single or several related genes or proteins, so it is very hard to know the whole change of DNA. Recently, some new technology such as comparative genome hybridization, microarray were adopted in the research NPC, but it still can not provide any information about the change level of protein. Another question is that information about protein modification, translocation and interaction with other proteins or bimolecules can not be known from DNA or mRNA. Thus, if the information on the whole changed protein can be integrated with the change information of DNA and mRNA, it will greatly help us to know more about NPC.Due to the especially anatomic localization and biological feature of NPC, the weight of NPC biopsy tissues normally is very small, it need to make the更多还原