【作者】 彭聪；

【导师】 李桂源；

【作者基本信息】 中南大学 ， 病理学与病理生理学， 2006， 博士

【摘要】 【BRD7基因的背景介绍】 BRD7是我室采用cDNA代表性差异分析的方法克隆的一个新基因。该基因在多数鼻咽癌组织及鼻咽癌细胞中表达缺失或明显下调。采用酵母双杂交系统(Two-hybrid system)筛选出6种与BRD7存在交互作用的蛋白，包括：溴区包含蛋白3(Bromodomain-containing 3 protein，BRD3)、溴区包含蛋白2(Bromodomain-containing 2 protein，BRD2)。通过特异性酵母双杂交技术和免疫共沉淀技术证实了BRD7基因能与BRD2、BRD3蛋白发生交互作用。将该基因转染到其表达下调的鼻咽癌细胞中，结果显示：软琼脂集落形成明显减少；裸鼠致瘤性检测发现移植瘤形成的时间明显延长，瘤体积减小，表明有着能够较强地抑制移植瘤生长的作用。上述结果说明该基因能部分逆转鼻咽癌细胞的恶性表型。运用流式细胞技术分析表明BRD7基因的高表达可阻滞鼻咽癌细胞的细胞周期G1-S的进程，并且发现BRD7基因能够通过对MEK1、p19、cyclinD1、cyclinE、E2F3、DP2、pRB等分子的调节参与细胞周期的调控。 生物信息学分析显示：BRD7基因含有一个重要的结构域—溴区更多还原

【Abstract】 [Background of BRD7]BRD7, a novel NPC-related gene, was isolated through cDNA representational difference analysis (RDA). GenBank accession number is AF 152604.Our previous studies showed that BRD7 was expressed at high level in normal nasopharyngeal epithelial tissues, but at low level in nasopharyngeal carcinoma biopsies and cell lines through NPC tissue array. Six BRD7-interacting proteins (BIPs) were identified by yeast two-hybrid system. These proteins included Bromodomain-containing 3 protein (BRD3) and Bromodomain-containing 2 protein (BRD2) which had been approved by immunoprecipitation(IP). Transfection of BRD7 into nasopharyngeal carcinoma cells could induce the reversion of some malignant phenotypes (data obtained from cell growth rate curve, soft agar colony formation, tumorigenic nude-mouse model, etc). And also,更多还原