【作者】 李春如；

【导师】 明亮； 李卫平；

【作者基本信息】 安徽医科大学 ， 神经精神药理学， 2006， 博士

【摘要】 抑郁症是一类病因复杂的精神疾病，已经成为不容忽视的医学和社会问题。目前，从中草药及微生物中寻找抗抑郁药已成为该领域的研究热点之一。虫草Cordyceps(Fr.)Link是一大类重要的昆虫病原真菌，也是受人瞩目的药物资源宝库。前期的研究结果表明，一些虫草类真菌代谢产物具有抑制MAO活性和抗抑郁作用。在此基础上，增加供试的虫草菌株并应用不同测定方法进行活性筛选，同时对供试虫草及其无性型进行鉴定和关系确证，以保证所用菌株的正确性。综合不同模型筛选的结果，优选出细脚拟青霉，再选择不同地理来源的10株细脚拟青霉进行复筛，优化复筛得到的菌株的培养工艺和发酵液提取工艺，按优化工艺培养和提取所得的样品即为BCPT。最后，应用慢性不可预知性应激模型研究BCPT对CUS大鼠抗抑郁作用及其机制，内容包括BCPT对CUS大鼠行为的影响，并从组织、细胞和基因等不同水平上研究BCPT抗实验性抑郁作用与组织过氧化、免疫功能调节和下丘脑CRH mRNA表达等方面的关系，探讨了可能的作用机制。本论文的主要研究结果如下： 1 虫草及其无性型鉴定 采用Kobayasi的分类系统和Dictionary of the Fungi(9th edition)的标准鉴定虫草及其无性型，并通过形态和分子手段及人工培养虫草子实体等方法，确证有性型与无性型两者的对应关系。鉴定虫草15种，无性型20种(供试菌株45株)，其中虫草及其无性型新种3个、新变种1个、新组合2个和中国新记录1个，即：根足被毛孢新种Hirsutella heteropoda、黄山被毛孢新种Hirsutella huangshanensis更多还原

【Abstract】 Depression is a mental disorder with complicated etiopathogenisis and has represented a major medical and social problem. At present, searching antidepressive agent from medical herbs and microbes has become a new focus. Cordyceps (Fr.) Link is a large group of entomogenous fungi and an important treasury of drug resources. Preliminary studies have shown that some metabolites derived from Cordyceps have obvious MAO inhibitory activity and antidepressant effect. In this paper, we selected more strains of Cordyceps using different activity-screening models. Meanwhile, the strains tested and its teleomorphic Cordyceps were identified. Paecilomyces tenuipes showed higher activity by different models. So, 10 strains of P. tenuipes from different geographical origins were screened using assays of DPPH-Microplate and MAO inhibitory activity repeatedly. RCEF0788 strain of P. tenuipes was chosen for further study based on the above results. Optimized technologies of culture and extraction of broth were studied for maximal MAO inhibition and bioactive component from broth of Paecilomyces tenuipes (BCPT) were obtained under the optimal conditions. At last, the effect of BCPT on behavior of chronic unpredictable stress (CUS) rats was investigated and the possible mechanisms of BCPT, including antioxidization, immunological更多还原