【作者】 余祖功；

【导师】 夏德全； 吴婷婷；

【作者基本信息】 南京农业大学 ， 基础兽医学， 2005， 博士

【摘要】 本文以网箱为样本单位，采用饵料添加或注射给药，每网箱雌、雄鱼数固定（饲料添加每组7尾雌鱼，23尾雄鱼；注射给药每组6尾雌鱼，18尾雄鱼；对照组和试验组均二个重复），自然养殖的方法，研究了几种生理调节剂对奥利亚罗非鱼雄、雌生长性能的影响，并通过相关激素水平及某些生理生化指标测定，探讨其可能的作用机制。研究内容如下： 一、盐酸半胱胺对奥利亚罗非鱼生长及相关激素水平、生化指标的影响 试验1．挑选大小相近的奥利亚罗非鱼270尾，随机分为3组，即对照组（基础日粮）、盐酸半胱胺低剂量（CSH）组(基础日粮+150mg.kg^-1CSH)、盐酸半胱胺高剂量（CSH）组(基础日粮+300mg.kg^-1CSH)，试验期为32d，分别于实验开始第1、15和32天测定体重、某些激素水平和生理生化指标的变化。结果表明，与对照组比较，高、低CSH剂量组雄鱼日增重分别提高40.3％、43.2％（P＜0.05）；特定生长率增加32.8％、28.7％（P＜0.05）；GH水平分别提高33％、46.7％；T₃水平分别提高28.5％、23％：睾酮水平分别提高35.3％、53.7％；雌二醇水平变化不明显；高、低CSH剂量组雌鱼日增重分别提高15.5％、24.5％；特定生长率增加8.7％、13.1％；GH水平分别提高34.4％、43.4％；T₃水平分别提高28.1％、32.3％；睾酮水平分别提高35.3％、53.7％；雌二醇水平变化不明显；高、低CSH剂量组平均饵料系数分别下降24.6％、24.8％（P＜0.05）；高、低CSH剂量组雌、雄鱼血糖、血钙水平变化不大；脲氮水平明显下降（P＜0.05），血清及肝脏抗氧化能力显著增强（P＜0.01）。 试验2．挑选大小相近的奥利亚罗非鱼，以网箱为样本单位，每网箱雌鱼（6）、雄鱼（18）固定，随机分为2组，即对照组（注射生理盐水）、注射CSH组（150mg／kg.B.WCSH），每周一次，连续使用四周，对照组和试验组均设2个重复。结果表明：与对照组比较，注射CSH组雄鱼日增重提高21.5％（P＜0.05），特定生长率增加22.2％（P＜0.05）；雌鱼日增重提高10.7％，特定生长率增加8.8％，CSH组平均饵料系数下降17.6％（P＜0.05）。注射CSH后3h、6h、12h、24h，GH水平雄鱼分别提高为15.2％、31.3％、45.2％、20.8％；雌鱼血清相应时间分别增加17.3％、17.3％、11.0％、19.5％；更多还原

【Abstract】 Seven experiments were conducted to evaluate the effects of several physiological regulators on growth performance of Oreochromis aureus and the working mechanism were also elucidated through testing correlative hormone and physiological and biochemical parameters with growth. The details are summarized as follows.Ⅰ. Effects of cysteamine hydrochloride on growth and correlative hormone and physiological and biochemical parameters with growth of Oreochromis aureus.Experiment 1. Two hundreds and seventy Oreochromis aureus with similar body weight were randomly divided into 3 groups with 23 male and 7 female in every net to evaluated the effects of cysteamine hydrochloride (CSH) on growth performance and Neuro-incretion mechanism involved. They were fed with basic diet (Group A), basic diet + 150 mg/kg CSH(Group B) and basic diet +300 mg/kg(Group C), respectively, for 32 days. On Day 1,15 and 32 after the feeding, all of Oreochromis aureus were sampled from each group for determination of Body Weight, serum hormone and physiological and biochemical parameters. The results showed that the average daily body weight gain (DBG) of the male fish from Group B and C significantly increased 40.3% and 43.2%(P<0.05), respectively, as compared with Group A; specific Growth Rate (SPR) significantly increased 32.8% and 28.7% (P < 0.05); the DBG and SPR of the female increased 15.5% and 24.5%, 8.7% and 13.1% (P>0.05), respectively. The concentrations of serum GH> T3 and testosterone in the male from Group B and C were elevated 33% and 46.7%, 28.1% and 32.3%, 35.3% and 53.7%, respectively; the concentrations of serum GH、T3 and testosterone in the female were elevated 34.4% and 43.4%, 28.1% and 32.3%, 35.3% and 53.7%, respectively. The concentrations of serum urea nitrogen (SUN) in male and female fish from Group B and C were significantly decreased (P<0.05), the serum and liver antioxidant capacity significantly increased (P<0.05). The concentrations of serum glucose, Ca2+ and E2 had no significant changes (P>0.05).更多还原