【作者】 陈秋；

【导师】 钱凯先；

【作者基本信息】 浙江大学 ， 细胞生物学， 2006， 博士

【摘要】 MGC13096是一功能完全未知的假设蛋白，它包含了一个PDCD2_C结构域。这个结构域同时也存在于PDCD2（Programmed cell death 2）蛋白中。GO（Gene ontology，基因功能分类体系）认为PDCD2和MGC13096都具有凋亡调节活性，参与凋亡过程。有文献对PDCD2是个凋亡相关蛋白提出了置疑。 通过对PDCD2的生物信息学分析表明：PDCD2在胸腺、淋巴结高表达；上游非翻译区有Elk-1，HEN1，HIC-1，HES-1，AP4，CDE／CHR等分化、增殖相关的转录因子结合；在生长、分化、感染类的基因表达谱中有较多的显著差异。而MGC13096则在结肠、肺、子宫颈、胃、肠等高表达；只发现在胰岛素抗性病人的基因表达谱中表达显著加强。 序列研究表明：在HEK293T、胃癌AGS细胞中PDCD2的两个转录本编码区与Genbank中的序列（NM₀02598、NM₁44781）相比均少了一段99bp的序列，Genbank接收号为：AY948416、AY948417。测序证实HEK239T、肝癌HepG2细胞中MGC13096的编码区序列与Genbank中的编码区序列（NM₀32346）完全一致。 用无血清、2％乙醇、4％乙醇、4％DMSO、8％DMSO等刺激HEK293T细胞发生凋亡的情况下，用流式细胞仪检测细胞凋亡情况，用荧光定量PCR方法分析MGC13096、PDCD2的基因表达情况，。无血清、2％乙醇刺激下DNA发生泄漏的凋亡细胞数没有显著变化，其更多还原

【Abstract】 There are only two isoforms of PDCD2 and MGC 13096 containing PDCD2_C domain in human genome. To study the role of PDCD2_C domain in apoptosis, the cDNAs of two isoforms of PDCD2 and MGC 13096 were cloned. The RT-PCR products (AY948416, AY948417) of PDCD2 from RNA of human embryonic kidney 293T (HEK293T) and gastric cancer AGS cell line lost common 99bp when compared with the sequences of NCBI database (NM 002598. NM 144781). The data of expression of PDCD2 and MGC 13096 genes in HEK293T cells which induced to undergo apoptosis by various treatments suggested that there wasn’t significant over-regulation of MGC 13096 gene and the over-expression of PDCD2 gene was not occurred universally. Perhaps PDCD2_C domain is not universally associated with apoptosis. GEO (Gene Expression Omnibus) search about MGC13096 (Hs.515344) and GDS161 record program indicated MGC13096 was significantly up-regulation on muscle of insulin-resistant patients compared with that of insulin-sensitive people (GDS161 record | GPL100 RC_T92180_at). DNA/flow cytometry analysis showed that MGC13096 overexpression would delay the cell cycle progression at S phase severely. On the other hand, although its containing PDCD2_C domain (Programmed cell death protein 2, C-terminal putative domain),更多还原