【作者】 冉波；

【导师】 于永利；

【作者基本信息】 吉林大学 ， 免疫学， 2006， 博士

【摘要】 口蹄疫(Foot-and-mouth disease，FMD)是一种高度传染性的动物疾病，主要发生于牛、羊、猪等偶蹄类畜牧动物。 口蹄疫的病原体是口蹄疫病毒(Foot-and-mouth disease virus，FMDV)。FMDV的衣壳蛋白VP1是其最主要的抗原蛋白，VP1刺激机体产生的抗体具有中和FMDV的作用。在本实验室前期工作的基础上，从应用的佐剂、免疫途径和疫苗的剂量三个角度对O型FMDV VP1表位重组蛋白(重组VP1epi)的免疫效果进行了系统的研究。 研究的结果表明：206油佐剂可以明显增强重组VP1epi的免疫原性；与其它免疫途径相比，经肌肉注射应用的重组VP1epi可刺激豚鼠产生高滴度的FMDV的特异性抗体；200μg是刺激豚鼠产生高水平抗体的重组VP1epi的适宜剂量；重组VP1epi诱生的IgG抗体可以有效地中和O型FMDV。 研究的结果还表明：以重组VP1epi包被作ELISA可以有效地检测由FMDV诱生的特异性抗体。 此外，也观察了含重组VP1epi包涵体免疫效果。 本文的结果提示，我们研制的O型口蹄疫病毒VP1表位重组蛋白疫苗可能成为一种新型的O型口蹄疫预防疫苗。更多还原

【Abstract】 Foot-and-mouth disease (FMD) is a highly contagious disease, which always inflicts cloven-hoofed animals such as cattle, sheep and pigs. Foot-and-mouth disease virus (FMDV) is the pathogen that causes this disease. Because of its dangerous infectivity and its impact upon agriculture, it was enlisted as number on the A list of contagious by OIE (Office International Des Epizooties). Among varies of farm animals, cattle, sheep and pigs are all sensitive to the infection of FMDV, among which cattle is the most sensitive one. Cattle and pigs display most serious symptoms while sheep only show very mild sub-clinical infection. Besides the economical loss caused by the death of farm animals, the reduced production of milk and meat during the epidemic will lead to large amount of loss. So the breakout of FMD always leads to the decrease of export and import of farm products of the country and great loss. The FMD breakout in England occurred in 2001, during which half a million animals were sacrificed and direct loss of 1.5 billion pound was caused. And it was showed in an investigation that the income of most of England farmers dropped 70% in recent 5 years.The major route of transmission of FMDV includes pathways through alimentary system, respiratory system, skin or mucosa. FMD may break out anytime of the year, especially at the end of autumn. The epidemic may aggravates during winter and abates in the spring. According to the past record, FMD always break out periodically, which means it may occur in the same region every few years.Foot-and-mouth disease is caused by the infection of Foot-and-mouth disease virus (FMDV). FMDV is a member of Aphthovirus genus of Picornaviridae. There are 7 sero-types of FMDV, namely, O, A, C, Southern African Territories 1 (SAT 1), SAT 2, SAT 3 and Asia 1. And there was no cross protection between these types. The genome of更多还原