【作者】 李志伟；

【导师】 杨更亮；

【作者基本信息】 河北大学 ， 分析化学， 2006， 博士

【摘要】 分子烙印技术(MIT)是从上世纪90年代新兴起的一门技术，以其极高的选择线性和分子识别性能，引起了广大科研工作者的兴趣。本文分别采用本体聚合、乳液聚合、原位聚合以及辐照引发聚合方式制备了烟酰胺、三唑醇、氟康唑、氨氯地平和M207等的分子烙印聚合物，并用于结构类似物和光学异构体的高效液相色谱分离分析，考察了不同色谱条件对分离的影响，实现了部分药物的水相分离；对本体聚合、乳液聚合和原位聚合的MIP进行了比较，结果发现原位聚合法制备的MIP具有制备简单、产率高和柱压降低等优点，适合作为快速、专一的分离介质用于分离分析；试验中发现热引发方式制备的氨氯地平MIP不能实现手性拆分，因此改为采用低温γ—射线辐照聚合的方法，实现了氨氯地平的手性分离，并且试验发现辐照聚合得到的MIP对于模板分子的识别能力更强，用于色谱分析时洗脱较慢，适合于在热引发中不易实现分离的化合物MIP分析，由于辐照聚合法由于不加入引发剂、聚合时间短和试验温度不受限制，因此适合于对温度敏感的化合物制备MIP；试验还考察了MIP的吸附性能，以烟酰胺MIP为例分别采用静态吸附和竞争吸附法对分子烙印聚合物的吸附性能进行研究，并将试验结果用Langumir和Freundlich模型进行模拟，两种研究方法的结果均显示MIP具有极高的吸附容量；由于原位聚合制备的MIP整体柱具有通孔、中孔和微孔结构，柱压降低，适合将得到的MIP整体柱用于痕量组分的富集分析，结果表明该方式富集效率高，样品过滤后直接进行富集分析，减少了样品处理的繁琐过程，提高了分析速度，大大缩短了痕量组分的分析周期，经济实用，操作简单，提高了分析检测灵敏度。更多还原

【Abstract】 Title of the Dr.degree’s Thesis: The Preparation Technology of Molecularly Imprinted Polymers and the ApplicationsIntroduction of the Author: Li Zhiwei, male, born in May of 1977, began his study as a PhD candidate under the supervision of Professor Yang Geng-Liang since 2003, specializing in analytical chemistry.Abstract: Robust molecular recognition elements with antibody-like ability to bind and discriminate between molecules or other structures can today be synthesized by molecular imprinting techniques. This includes the synthesis of cross-linked polymers in the presence of templates which may be small molecules, biological macromolecules, microorganisms or whole cells. Removal of the template from the formed polymer thus generates a structure complementary to the template structure or to an analogous structure. There are three parts which cover some aspects of the synthesis, adsorption isotherm and applications of the imprinted materials. The paper is divided into seven chapters.The first chapter discussed the histrical perspective and fundamental aspects on the synthesis, recognition and application of the imprinted polymers. The next four chapters contain the bulk, emulsion, in-suit polymerization with high temperature and y-ray initiation methods. The sixth chapter investigated the adsorption isotherm of the nicotinamide imprinted polymers. In the last chapter, the applications of the MIP on the on-line preconcentration are concluded.The MIPs which have been synthesized by different methods are used to separate template, its analogues and chiral compounds. Nicotinamide, triadimenol, fluconazole, M207 and amlodipine are used as the templates to synthesize the MIPs, which are used to investigate the separation, Langmuir and Freundlich adsoption isotherms of the drugs above. The on-line pre-concentration method fits for trace analysis.更多还原