【作者】 王华；

【导师】 姚裕家；

【作者基本信息】 四川大学 ， 儿科学， 2005， 博士

【摘要】 第一部分:新生鼠缺氧缺血性脑损伤脑组织光镜及电镜改变 目的:了解新生大鼠缺氧缺血脑损伤时,脑组织病理学及电镜改变。 方法:7日龄SD大鼠经右侧颈总动脉结扎,吸入8%O₂、92%N₂混合气体行低氧处理3h,以建立缺氧缺血性脑损伤（HIBD）的动物模型。用HE染色观察脑组织病理变化,Nissl染色分析神经元数量变化,电镜检查超微结构改变。 结果:（1）缺氧缺血后6h-12h,右侧脑组织肿胀,神经元水肿;24h神经元水肿加重,并同时出现神经元坏死;72h出现明显的脑梗塞。（2）缺氧缺血后12h、24h及72h组神经元数量递减,均与对照组有显著性差异（P均<0.05）。（3）缺氧缺血后6h电镜观察脑组织线粒体形态有明显改变。 结论:结扎新生SD大鼠右侧颈总动脉及吸入8%O₂行低氧处理3h,经光镜检查脑组织,证实符合HIBD的病理改变,即神经元水肿及坏死,且神经元数量递减,同时超微结构也发生改变。表明本研究HIBD动物模型制作成功。更多还原

【Abstract】 The first part: The light and the electron microscopechanges of brain in neonatal rat with HIBDObjective:To detect the pathological and infrastructure changes of brain inneonatal rat with hypoxic-ischemic brain damage (HIBD). Methods:The 7 day-old SD rats were subjected to the ligation of right carotid artery(ischemia),and then they were put into a box full with 8% oxygen and 92% nitrogen for 3 hours(hypoxia).The light microscope was used to observe the brain pathological changes and the electron microscope was used to detect the brain ultrastructural changes.Results:The changes of right side brain of rat had significantly pathological changes by 6-24h of HIBD and infarction of brain tissue had been observed by 72h group of HIBD compared with that in the control group.The number of neurons is significantly decreased in damagedgroups in the SD neonatal brain tissue within 6h ,at 12h and 24h, compared with the control groups P<0.05, and then gradually decreased.The expression of NgR protein was significantly increased in HIBD groups in the SD neonatal brain tissue within 6h, at 24h and 72h, compared with the control groups PO.05? and then gradually decreased.The expression change trends of NgR mRNA are identical with that of NgR protein.Conclusions:Above results suggest that the increasing of NgR mRNA and NgR protein may be correlated with the hypoxic-ischemic injury and may inhibit the neuron regeneration.The decrease in expression of NgR mRNA and protein may be correlated with the loss of nerve cell. The change trend of NgR and Nogo-A is identical.更多还原