【作者】 李平；

【导师】 马润宇；

【作者基本信息】 北京化工大学 ， 化学工程， 2005， 博士

【摘要】 我国中药材资源丰富,有巨大的发展潜力。许多中药材中的多糖能通过增强或调节机体的免疫功能来消除体内病因,以实现抗肿瘤等多种药理功能,是中药发挥独特疗效的重要物质基础之一。论文第一章至第四章以我国中草药基地四川产山茱萸为原料,经提取、分离、纯化得到两种新的山茱萸多糖,并通过化学分析及仪器分析对这两种山茱萸多糖的结构、理化特性及生物活性进行了研究,为山茱萸多糖的保健品及药品的开发提供重要的理论基础。黄酮类物质是植物中的一类重要有效成分,具有多种药理功能,树脂吸附法是目前分离黄酮类物质的主要方法。本文第五章利用树脂吸附法分离沙棘黄酮和茶多酚,确定了树脂类型、吸附—洗脱工艺条件,并尝试将溶解度参数规律应用于洗脱剂的选择,发现了溶解度参数之差与洗脱率之间的对应关系,从而对洗脱剂的选择起到一定的指导作用。 (1)通过单因素实验及正交实验对山茱萸多糖提取过程工艺条件进行优化,确定山茱萸多糖水提取的最佳工艺参数,并系统地用水提取→脱蛋白→DEAE-纤维素柱分离→Sephadex G-200柱纯化的工艺,从山茱萸中分离得到PFCAⅢ;将山茱萸水提取残渣经过碱液提更多还原

【Abstract】 There are abundant herbal medicines in China, which has great potential to be developed. Polysaccharide is the basis for its biological activity and pharmacology functions such as anti-oxidation and anticancer. In chapter 1 to chapter 4 of this dissertation, separation, characteristics and biological activities of polysaccharides from Fructus corni were investigated. Flavones is another important effective components in herbal medicines, which is often separated with macroreticular resins. In chapter 5 of this dissertation, separation process of total flavones of Hippophae and tea polyphenols with macroreticular resins were studied, and a method of choosing desorption solvent using solubility parameters was put forward theoretically and experimentally.(1) Several factors that affecting the extraction process were studied through single factor and orthogonal experiments. The optimal technological conditions of water extraction from Fructus corni were decided: the temperature being 80°C, the extraction time being 2 hours, the ratio of solid material to water being 1 : 16, the granularity of Fructus corni being 300-450 μm, the times of extraction at the same ratio of solid material to water being 4, and the times of clearing protein being 6. A purified polysaccharide PFCAIII was prepared with process as the following: water extracting → DEAE-cellulose chromatographic fractionating → sephadex chromatographic purifying. Another purified polysaccharide PFCC I was prepared with process as the following: extracting with sodium hydroxide → DEAE-cellulose chromatographic fractionating→ sephadex chromatographic purifying.更多还原