【作者】 华金双；

【导师】 孙忠人；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2005， 博士

【摘要】 目的:揭示艾灸预处理的预防性脑保护作用机制,为缺血性脑血管疾病的防治开辟新途径。 方法:78只健康Wistar雄性大鼠,随机分为5组:正常对照组、假手术组、脑缺血组、脑缺血预处理组、艾灸预处理组,各组又分为24、48、72小时三个时间点。大脑皮层、海马CA₁区HE染色观察组织形态学变化、电镜观察组织超微结构、原位末端标记法检测神经细胞凋亡、免疫组化和原位杂交检测Bcl-2、Bax、HSPmRNA和蛋白表达、黄嘌呤氧化酶法测定SOD活性、硫代巴比妥酸法测MDA含量、干湿法测定脑组织水分含量。 结果:① 艾灸预处理同缺血预处理一样,能明显减少缺血后神经细胞凋亡数目,与脑缺血组比较具有显著性差异。② 艾灸预处理后Bcl-2、HSPmRNA和蛋白表达明显增多,且表达高峰提前,与脑缺血组相比,差异显著。③ 艾灸预处理后BaxmRNA和蛋白表达变化不明显,与脑缺血组比较无显著性差异。④ 艾灸预处理后SOD活性明显增强,24小时尤其明显;MDA含量显著下降,与脑缺血组比较具有显著性差异。⑤ 艾灸预处理后脑组织水分含量较脑缺血组明显减少,具有显著性差异。 结论:① 艾灸预处理可以延缓或者阻断缺血性卒中的发展进程,并且具有提高脑组织在缺血时对一系列代谢紊乱的耐受能力,增加神经元的存活率,改善预后的作用。② 艾灸预处理可以抑制全脑缺血大鼠大脑皮层、海马CA₁区神经元凋亡。③ 艾灸预处理可通过上调细胞凋亡基因Bcl-2mRNA和蛋白表达而抑制缺血脑组织神经细胞凋亡。④ 艾灸预处理的预防性脑保护作用可通过促进HSP70mRNA、HSP70蛋白表达高峰提前实现。⑤ 艾灸预处理可通过内源性抗氧化物质的增加对缺血缺氧脑组织起保护作用。更多还原

【Abstract】 Object: To reveal the mechanism of neuroprotective effects of moxibustion preconditioning, break fresh ground for cerebrovas -cular disease.Measure: 78 rats were divided into five groups randomly in the experiment: normal control group, sham-operation group, ischemic group, ischemic preconditioning group, moxibustion preconditioning group. HE staining to observe change of histomorphology. electron microscope to observe ultrastructure, TUNEL method to observe apoptosis state, immunohistochemistry and ISH technique to detect Bcl-2 Bax HSPmRNA and protein expression, xanthine oxidase method to detect the active of SOD, Thibabituric Acid to dect the amount of MDA, wet-dry comparison to detect moisture content.Result: (1) Moxibustion preconditioning can decrease cell apoptosis as ischemic preconditioning which showed remarkable difference compared with ischemic group. (2)Bcl-2 HSPmRNA and protein expression increase significantly after moxibustion preconditioning. The peak appeared ahead of time which was remarkable difference compared with ischemic group. (3)BaxmRNA and protein expression showed no remarkable difference compared with ischemic group.(4)The active of SOD increase significantly, especially 24h after moxibustion preconditioning. The amount of MDA decrease significantly which showed remarkable difference compared with ischemic group. (5)Moisture content of brain tissue is decreased after moxibustion preconditioning which showed remarkable difference compared with ischemic group.Conclusion: (1)Moxibustion preconditioning cam delay or abolish the further advance of ischemic stroke and increase the更多还原