【作者】 王伟；

【导师】 宋惠民；

【作者基本信息】 山东大学 ， 外科学， 2004， 博士

【摘要】 目的:通过建立大鼠颈部心脏移植模型,观察心脏移植后立即行供体淋巴细胞或淋巴细胞提取物注射对移植心脏存活的影响,以及诱导免疫耐受的证据。进而探讨一种新的更有临床应用价值的诱导免疫耐受的方法。 材料和方法:Wistar大鼠60只,SD大鼠50只。受体采用健康SD大鼠,雌雄不限,体重250—350g,购自中科院上海实验动物中心。供体采用健康Wistar大鼠,雌雄不限,体重150—250g,购自山东大学实验动物中心。普通饮食喂养。实验动物分为5组: A组:供体与受体大鼠各5只。受体不做任何预处理。 B组:供体与受体大鼠各15只。受体移植术当日开始用CsA10mg/kg,隔日一次,共8—10次,腹腔注射。于术后5天、10天各处死5只受体,取出供心,为B1组、B2组,剩余5只于受体死亡后取出供心,为B3组。 C组:供体与受体大鼠各15只。受体手术过程中给供体淋巴细胞静脉注射5—10×10~7个,手术当日开始用CsA10mg/kg,隔日一次,共8—10次,腹腔注射。于术后5天、10天、25天各处死5只受体,为C1、C2、C3组。 D组:供体与受体大鼠各15只。受体手术过程中给供体淋巴细胞提取物静脉注射,手术当日开始用CsA10mg/kg,隔日一次,共8—10次,腹腔注射。于术后5天、10天、25天各处死5只受体,为D1、D2、D3组。 E组:供体与受体大鼠各5只。供受体均为Wistar大鼠,受体不经任何处理。 颈部心脏移植术:10%水合氯醛麻醉受体大鼠,将受体右侧颈外静脉及颈总动脉分离,远心端结扎,近心端套管。然后麻醉供体大鼠,腹部正中切口。全身肝素化后自腹主动脉取血5—10ml留备淋巴细胞用。向上延伸切口剪开胸骨,暴露降主动脉,用5ml空针经降主动脉快速注射0℃冷停跳液10ml使心脏停跳。切断并结扎下腔静脉、右上腔静脉、左上腔静脉。切断降主动脉,提起降主动脉断端,延脊柱前方向上游离整个心脏、肺、食道。修剪供心,将肺动脉套于颈外静脉套管上,更多还原

【Abstract】 Objective: Creat the cervical heart transplantation model between Wistar and SD rats. To induce the donor specific immunologictolerance, the recipient was injected with donor lymphocyte and extract of donor lymphocyte after the heart was transplanted. Cyclosporin-A was injected into abdominal cavity of recipient after operation. Observe the survival time of donor heart and grade of heart rejection.Material and Methods: Wistar rats were used as the donor and SD rats as recipients. Body weight of Wistar range from 150 to 250 grams, and the SD range from 250 to 350 grams. The rats are devided into 5 groups. Group A included 5 donors and 5 recioients. Group B included 15 donors and 15 recipients. Group C included 15 donors and 15 recipients. Group D included 15 donors and 15 recipients. Group E included 5 Wistar as donor and 5 Wistar as recipient. 10% chloral hydrate was injected into the donor’s abdominal cavity to anesthesia it. Separate the right external jugular vein and common carotid. Deligation the axifugal terminal and cannula the other terminal. Anesthesia the recipient, abdominal median incision. The recipient was heparinizationed all over the body. 5 ml blood was drew from recipient abdominal aorta. Cut the sternum. Inject 10 ml 0℃ beat-ceasing fluid through descending aorta to cease the beat of donor heart. Cut off and deligation the inferior vena cava, left superior vena cava. Cut off descending aorta. Separate the heart, lung, esophogus as a whole part along the spinal column. Clip the donor heart and connect the pulmonary artery of donor heart with jugular vein of recipient, connect the aorta of donor heart with common carotid of recipient. Open the vascular clamp and the donor heart rebeat slowly. Several seconds later the rhythm became irregular and fast. Group C recipient was injected with donor lymphocyte 5 × 10~7 through caudal vein after the donor heart was transplanted. Group D recipient was injected with donor lymphocyte extract 0.5 ml through caudal vein. Grading of heart更多还原