【作者】 谭朝阳；

【导师】 尤昭玲；

【作者基本信息】 湖南中医学院 ， 中西医结合妇产科学， 2004， 博士

【摘要】 为了筛选SQ复方（由三七、茜草组成）的有效成分，采用置Cu-IUD家兔动物模型，以6-酮前列腺环素(6-K-PGF_1α)和血栓素B₂（TXB₂）的含量和比值、组织型纤溶酶原激活剂（t-PA）的含量、血液流变学、子宫内膜浅血管数等为指标，进行了多次动物试验研究，结果显示： 1．置器家兔动物模型发生了病理改变 表现为生化指标的变化、血液流变学的异常、子宫内膜充血等，SQ复方具有治疗作用，可以使这些异常变化得到改善。 2．三七、茜草单独治疗亦有作用，但效果不如三七、茜草合用。 3．三七中主要有效成分是三七总皂苷、三七素，茜草的有效成分是大叶茜草素，均能使异常变化的生化指标及血液流变学指标得到改善，而提取后的三七剩余物和茜草剩余物则基本无治疗作用。 4．三七总皂苷、三七素、大叶茜草素对指标的改善均有显著性影响，其最佳处方配比为三七总皂苷240mg、三七素24mg、大叶茜草素100mg（一日剂量）。 经实验研究，建立并优选了三七总皂苷、三七素、大叶茜草素的提取工艺，其结果如下： 1．茜草经乙醇加热回流提取后，用D₁₀₁大孔吸附树脂进行纯化，所得大叶茜草素粗品含量较高，达70％以上。 2．三七以乙醇渗漉提取后，用D₁₀₁大孔吸附树脂进行纯化，所得三七总皂苷含量达90％以上，杂质较少。 3．含三七总氨酸的水洗脱液经732强酸型阳离子交换树脂柱，得三七总氨酸，再经CM Sephadex C-25柱分离，得到的三七素粗品，经小鼠尾静脉切断实验，三七素粗品能明显缩短止血时间。更多还原

【Abstract】 In order to confirm the effective components in SQ complex prescriptions which was composed of Radix notoginseng and Radix rubiae, an animal model of Cu-IUD-wearing rabbits was established and 6-K-PGF1, TXB2, t-PA, hemorheology markers, endometrium micro vessel count were used as the screening markers. Several animal tests were taken. The results showed :l.The animal model of rabbits with Cu-IUD-wearing had pathological alteration with manifestates of biochemical marker alterations, hemorrheologic abnormality, endometrium hyperemia etc. SQ complex prescriptions had therapeutical effects, could ameliorate these abnormal alterations.2.Radix notoginseng and Radix rubiae had therapeutical effects when apply alone, but their effects had not as good as combinational apply.3. The chief effective components of Radix notoginseng were Panax notoginseng saponin (PNS) and dencichine. The effective component of Radix rubiae was mollugin. All of these three effective components could ameliorate abnormal hemorheology alterations and biochemical marker alterations. The rest extract of Radix notoginseng and Radix rubiae had fewertherapeutical effects.4. PNS, dencichine and mollugin all had significant effects on ameliorate abnormal alterations. The best prescription matching was PNS 240 mg, dencichine 24 mg, mollugin 100 mg (one day)The extration process of PNS, dencichine and mollugin were established and optimized by several tests. The results showed :1 .Mollugin was extracted with ethanol from Radix rubiae by heating reflux and purified with DIOI macroporous resin, its content up to 70%.2.PNS was extracted with ethanol from Radix notoginseng by infiltrating, and purified with D101 macroporous resin, its content up to 90%.3.The coarse dencichine was isolate from the total ammo acid with CM Sephadex C - 25, the later was isolate from the Radix notoginseng solution with 732 cation exchange resin. The coarse dencichine could apparent shorten hemostasia time by cutting mice caudal vein tests.更多还原