【作者】 刘军；

【导师】 孙汉文； 罗国安；

【作者基本信息】 河北大学 ， 分析化学， 2002， 博士

【摘要】 电极是研究生物分子电荷传递行为的有效平台。以生物分子间特异的生物亲和作用为基础而构置的生物亲和电极并应用于生物电化学领域，这不仅有利于生物分子的检测、有助于深入认识生物分子的结构与功能间的关系，并且对进一步开发仿生器件、丰富修饰电极的理论及技术也具有重要意义。本文通过多种途径构置了生物亲和电极，并主要应用于以下几方面生物分子的分析。采用自组装技术和化学吸附手段，以石墨、金电极为基础构置的生物亲和电极应用于电化学分析并结合光谱技术对多巴胺、硫堇、大黄素与DNA分子间的相互作用进行了研究，实验同时表明，交流阻抗技术是研究小分子与DNA分子间相互作用的快速、简便的方法；首次利用纳米SnO₂、纳米碳管等构置的生物亲和电极对多巴胺、抗坏血酸进行了分析，复合纳米SnO₂修饰石墨电极对NO分子具有特殊的识别作用，对生物体内的NO进行了测定；利用银胶和自组装技术得到了抗人白介素-2单克隆抗体电极，成功地监测和研究了抗体电极与抗原的免疫反应，为无标记免疫反应的监测提供了条件；将明胶酶底物直接定向组装在金电极上，得到了对明胶酶具有生物亲和作用的明胶电极，通过观察电化学阻抗谱的变化，对明胶酶与明胶的结合特点进行了研究，为研究酶开辟了另一条途径。更多还原

【Abstract】 Electrodes are available experimental platform to study the electron transfer behaviors of biomolecules. Based on the specific interactions among the biomolecules, the bio-affinity electrodes are fabricated and applied in bioelectrochemistry. It will be of great help for bioassay, further understanding the relationship between the biological function of some biomolecules and their structures. Besides, it may also be important significance to enrich the theory and techniques in the filed of chemically modified electrodes. In the present dissertation, appropriate and modified electrodes are employed to analysis biomolecules. Self-assembled technique and chemical adsorption approaches are employed to modify graphite and gold electrodes, the combination of electrochemical studies with spectroscopic measurements on the interactions between small molecules and DNA, such as thionine, dopamine, and emodin etc.. Electrochemical impedance methods can be used as an effective method for the interactions between DNA and small molecules. Nanosized materials modified graphite electrodes exert excellent selectivity and sensitive in analysis neural molecules, especially, Nafion-SnO2 modified graphite electrodes has electrochemical recognition of NO molecules, the concentration of NO in rabbit’s brain is detected. Antibody proteins were immobilized on cysteamine self-assembled gold electrode by silver colloid. The antigen of Interleukine-2 is detected. The analysis of the electrochemical impedance behavior of such layers can lead to a sensitive immunosensor for the direct detection of the antigen/antibody interaction. An obvious electrochemical behavior was obtained when a gold electrode assembled gelatin by putting it into gelatinase solutions, including medium culturing cancer cells. The results prove the brilliant future for the development of a rapid electrochemical method on gelatinase analysis.更多还原