针灸、穴位注射对肝纤维化大鼠肝功能及细胞外基质影响的研究

【作者】 陈旭军；

【导师】 罗永芬；

【作者基本信息】 成都中医药大学 ， 针灸学， 2002， 博士

【摘要】 目的：从针灸、穴注对肝纤维化大鼠肝功能及细胞外基质影响角度探讨针灸、穴注抗肝纤维化的疗效及其作用机制。 方法：用CCL₄诱导大鼠肝纤维化模型，以秋水仙碱为对照，观察针灸、穴注对大鼠血清ALT、Alb以及肝组织Ⅰ、Ⅲ型胶原、FN、LN、MMP、UPA、TIMP的影响。 结果：与模型组相比，针灸、穴注组大鼠血清ALT降低，Alb升高；肝组织Ⅰ、Ⅲ型胶原、FN、LN明显减少；MMP、UPA含量明显增加，TIMP明显减少。 结论：针灸、穴注有防治肝纤维化作用，其作用机制与针灸、穴注改善肝功能，减少胶原等ECM沉积，通过MMP／TIMP途径促进ECM降解有关。更多还原

【Abstract】 Objective: To study the effects and its mechanism of Acupuncture-moxibustion and acupoits-injection on anti-hepatofibrosis from the way of liver function and ECM.MethodsrHepatofibrosis model was induced in rats by CCL and meanwhile treated by Acupuncture-moxibustion and acupoits-injection with Colchicines as control, serum level of ALT, albumin and C-I, C-III , FN, LN, MMP , UFA , TIMP in liver tissue were observed.Results:Compared with model group , Serum ALT were lower and albumin higher in Acupuncture-moxibustion and acupoits-injection group; C-I, C-III, FN, LN in liver tissue were lower; MMP, UFA more and TIMP less.Conclusion: Acupuncture and acupoits-injection have preventive and therapeutic effects on hepatofibrosis, and its mechanism is related to improving liver function .reducing the deposition of ECM such as collagen , increasing the degradation of ECM by the way of MMP/TIMP.更多还原