【作者】 付玉杰；

【导师】 祖元刚；

【作者基本信息】 东北林业大学 ， 植物学， 2002， 博士

【摘要】 本文对采用超临界二氧化碳萃取技术从甘草中萃取甘草黄酮，柱层析技术分离纯化甘草黄酮，超临界后固形物提取纯化甘草酸的工艺进行了深入而系统的研究。 1．全面而系统地确定了测定甘草黄酮含量的UV分光光度法和甘草酸含量的HPLC分析方法，确定了甘草黄酮提取液中监测甘草甙、异甘草甙、异甘草素、和甘草次酸含量的HPLC分析方法。 2．对影响超临界二氧化碳萃取甘草黄酮的传质过程和溶解度因素进行了研究，确定了工艺参数。实验结果表明超临界二氧化碳萃取甘草黄酮收率1.35％，含量32.45％，比常规的索氏提取甘草黄酮在收率和质量上具有明显的优势。 3．分别对柱层析和大孔吸附树脂分离纯化甘草黄酮进行了研究，确定了柱层析分离纯化甘草黄酮的分离条件，甘草黄酮收率1.12％，含量55％。 4．对超临界萃取后固形物提取纯化甘草酸的工艺进行了研究，通过影响因素试验和正交试验得出最佳工艺条件，甘草酸铵收率1.45％，纯度95％。 本工艺充分利用了甘草植物资源，实现了投入一批原料，经过一条工艺路线，将甘草中甘草黄酮和甘草酸有效地提取、分离、纯化，为研制甘草的高质量、高附加值药品提供了理论和实践基础。更多还原

【Abstract】 Deeply and systematically researches had done to the extraction flavonoids from licorice using supercritical carbon dioxide,purification flavonoids through silica chromatography and extraction,purification glycyrrhiza acid from the licorice residue.1 .Decided the HPLC analysis conditions of the flavonoids and glycyrrhiza acid in general and system way. Checked the contents of liquiritin,isoliquiritin,isoliquiritigenin,glycyrrhiza acid and glycyrrhetinic acid during the process.2.Studied the transferring substance characteristics and solubility factors during supercritical carbon dioxide extraction flavonoids ,obtained the better process condition. The yield of flavonoids was 1.35% and the content was 32.45%,that was much more better than traditional method.3.Studied the purification of flavonoids through silica and large diameter resin chromatography,obtained the better silica chromatography condition of purify flavonoids,the yield of flavonoids was 1.12% and the content was 55%.4.Studied the extraction and purification of glycyrrhiza acid from licorice residue,obtained the better process condition through effect factors and orthotropic experiments,the yield of monoammonium glycyrrhizinate was 1.45% and content was 95%.This process used the licorice resource sufficiently,obtained flavonoids and glycyrrhiza acid in one process through one group of raw material ,and provided the strong base of theory and practice to develop licorice medicine with high quality and profits.更多还原