【作者】 金泽；

【导师】 孙申田；

【作者基本信息】 黑龙江中医药大学 ， 针灸推拿学， 2002， 博士

【摘要】 本实验采用6-羟基多巴胺(6-OHDA)注入纹状体尾壳核(CPU)的方法，在纯种(Wister)大鼠身上复制出偏侧帕金森病(PD)模型，借助动物立体定向仪将针灸针刺入丘脑底核(STN)，将电针治疗仪的正极连接在刺入STN的针灸针上，负极接在临时刺入大鼠背上的针灸针，予以电针治疗并观察治疗后PD大鼠行为改善程度，处死后检测损毁侧黑质或纹状体各项指标的变化。实验结果表明：①电针治疗后PD模型大鼠旋转次数明显减少；②电针治疗后PD大鼠黑质纹状体病理学检测神经细胞数略有增多，细胞变性减轻，镜下见阳性凋亡细胞数比模型组明显减少，差异显著(P＜0.05)，TH阳性细胞表达增多，与模型组比较差异显著(P＜0.05)；③电针治疗后PD大鼠纹状体DA、HVA、DOPAC含量较模型组明显增加，二者相比差异极显著(P＜0.01)；④与模型组相比，电针组MDA含量和NOS的活性显著下降而GSH的含量及GSH-Px的活性显著升高(P＜0.01)，说明电针治疗后能够使PD大鼠脑内抗氧化酶系统得到调整并恢复平衡，使机体免受过量活性氧的攻击，减轻多巴胺能神经元损伤；⑤电针治疗后PD大鼠Glu含量显著降低(P＜0.01)，Asp含量明显减少(P＜0.05)。提示电针治疗后使EAA含量降低，从而减轻对黑质－纹状体DA能神经元毒性作用。 从我们的实验结果可以看出，针刺PD模型大鼠STN后大鼠不但没有象传统针灸学说中所说的“针刺入脑立死”，而且治疗后的诱发旋转行为和各项观测指标明显改善，说明针刺脑内核团治疗PD的方法是可行的、有效的，它填补了针刺脑内核团治疗PD模型大鼠的实验空白，为临床治疗PD提供了理论和实验基础。更多还原

【Abstract】 This research successfully duplicates the model of PD in one side of pure Wister mice’s body by injecting 6-OHDA into CPU. We inserted the needle into the animal’s STN with the help of The Rat Brain in Stereotaxic coordinates, lining the electro acupuncture positive pole with the needle buried in STN ,lining the negative pole with the needle inserted in back. The rotation rate Was measure as an index of behavior recovery after treatment of acupuncture.and checked out the varieties of data about destroying the SN or CPU after the rat were killed. The research showed as follows. The first : The rotation rate of PD rat model is sharply reduced after the treatment. The second: We found out a bit of more nerve-cells less the losing activity cells and less the positive dying-cells than the controls in SN and CPU examination of pathology. (P<0. 05). Another result is that there were sigificance difference in expressing TH masculine cells as the electro acupuncture groups compared with the controls (P<0. 05). The third :The contenttration of DA HVA DOPAC in PD rat’s CPU is more markedly increased before treatment than the controls’ .The forth:The activity of MDA and NOS decreased in treatment groups (P<0. 01), but the concentration of GSH-Px the activity of GSH-Px markedly increased(P<0. 01). It indicates that the electro acupuncture treatment can regulate imbalance of oxygen free redical in the PD rat’s brain and keep the body from injuring of excessive activating oxygen and deprive the injuring of DA-nerve .The fifth: After treatment the Glu is lower (P<0.01), so is ASP (P<0.05). It turned out the treatment can help the DA-nerve improve its resistance after stimulating the STN by electricity that can make EAA less.We draw a conclusion that inserte the needle into the STN of PD models achieves a good success which has a good therapatic and has many improving data . On such researchwe made a good example. Before us, there was an old concept that "As soon as the needle is inserted into the brain, the man will die "accordin-g the TCM theory. But now we can cancel it . So we made a practical and theoretical base on the clinical treatment, which cures the PD.更多还原